You are here: Home Published Research QseC controls biofilm formation of non-typeable Haemophilus influenzae in addition to an AI-2-dependent mechanism.

Can M Unal, Birendra Singh, Christophe Fleury, Kalpana Singh, Luis Chávez de Paz, Gunnel Svensäter, and Kristian Riesbeck (2012)

QseC controls biofilm formation of non-typeable Haemophilus influenzae in addition to an AI-2-dependent mechanism.

International journal of medical microbiology : IJMM, 302(6):261–269.

Non-typeable Haemophilus influenzae (NTHi) is a common pathogen associated with diseases such as acute otitis media or exacerbations in patients with chronic obstructive pulmonary disease. The biofilm-forming capability substantially contributes to the persistence of NTHi. However, the regulation of biofilm formation is not completely understood. Quorum sensing regulated by autoinducer-2 produced by luxS is until now the only described regulatory mechanism. In this study, we show that the two-component signalling system QseB/C is involved in the biofilm formation of NTHi in vitro. An isogenic NTHi mutant of qseC (Hi3655KR2) showed a significant decrease in biofilm formation under static and semi-static conditions as assessed by crystal violet staining. In addition, under constant flow conditions, Hi3655KR2 formed less biofilm after 48 h. The biofilm defects were irrespective of autoinducer-2 levels. Hence, here we suggest for the first time a regulatory circuit in NTHi, which controls biofilm formation by mechanisms other than or in addition to luxS-dependent factors.

Amino Acid Sequence, Bacterial Proteins, Biofilms, Carbon-Sulfur Lyases, Culture Media, Escherichia coli, Escherichia coli Proteins, Gene Expression Regulation, Bacterial, Gene Knockout Techniques, Genes, Bacterial, Genetic Complementation Test, Gentian Violet, Haemophilus influenzae, Homologous Recombination, Homoserine, Lactones, Luminescent Measurements, Microbial Viability, Molecular Sequence Data, Protein Structure, Tertiary, Quorum Sensing, Sequence Homology, Amino Acid, Time Factors
Amino Acid Sequence, Bacterial Proteins, Biofilms, Carbon-Sulfur Lyases, Culture Media, Escherichia coli, Escherichia coli Proteins, Gene Expression Regulation, Bacterial, Gene Knockout Techniques, Genes, Bacterial, Genetic Complementation Test, Gentian Violet, Haemophilus influenzae, Homologous Recombination, Homoserine, Lactones, Luminescent Measurements, Microbial Viability, Molecular Sequence Data, Protein Structure, Tertiary, Quorum Sensing, Sequence Homology, Amino Acid, Time Factors
 
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