You are here: Home Published Research Oropharyngeal colonization by nontypeable Haemophilus influenzae among healthy children attending day care centers.

Carmen Puig, Sara Marti, Ana Fleites, Rafael Trabazo, Laura Calatayud, Josefina Liñares, and Carmen Ardanuy (2014)

Oropharyngeal colonization by nontypeable Haemophilus influenzae among healthy children attending day care centers.

Microbial drug resistance (Larchmont, N.Y.), 20(5):450–455.

Haemophilus influenzae colonizes the upper respiratory tract and can spread causing otitis and sinusitis. This work aimed to study the oropharyngeal carriage rate in healthy <5-year-old children attending day care centers in Oviedo, Spain in two consecutive years (January to March 2004-2005). The carriage rate was 42% (400/960) and highly variable among centers (range, 12% to 83%). Isolates were mainly identified as nontypeable H. influenzae (NTHi, 99%). Epidemiologically, 127 different genotypes were identified by PFGE with a minimum of two genotypes per center. One hundred fourteen children (12%) were included in both studies and none of them harbored the same strain over a period of time. The isolates only showed resistance to cotrimoxazol and ampicillin, presenting a shift in the level of ampicillin reduced susceptibility, showing a predominance of PBP3 mutations in 2004 and a predominance of β-lactamase production in 2005. This study proved the great genetic variability of NTHi isolates that present similar genotypic patterns in both years with no long-term carriage of the same strain.

Bacterial Typing Techniques, Carrier State, Child Day Care Centers, Child, Preschool, Electrophoresis, Gel, Pulsed-Field, Female, Genetic Variation, Genotype, Haemophilus Infections, Haemophilus influenzae, Humans, Infant, Male, Mutation, Oropharynx, Spain
Bacterial Typing Techniques, Carrier State, Child Day Care Centers, Child, Preschool, Electrophoresis, Gel, Pulsed-Field, Female, Genetic Variation, Genotype, Haemophilus Infections, Haemophilus influenzae, Humans, Infant, Male, Mutation, Oropharynx, Spain
 
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