You are here: Home Published Research A PCR-high-resolution melt assay for rapid differentiation of nontypeable Haemophilus influenzae and Haemophilus haemolyticus.

Janessa Pickering, Michael J Binks, Jemima Beissbarth, Kim M Hare, Lea-Ann S Kirkham, and Heidi Smith-Vaughan (2014)

A PCR-high-resolution melt assay for rapid differentiation of nontypeable Haemophilus influenzae and Haemophilus haemolyticus.

Journal of clinical microbiology, 52(2):663–667.

We have developed a PCR-high-resolution melt (PCR-HRM) assay to discriminate nontypeable Haemophilus influenzae (NTHi) colonies from Haemophilus haemolyticus. This method is rapid and robust, with 96% sensitivity and 92% specificity compared to the hpd#3 assay. PCR-HRM is ideal for high-throughput screening for NTHi surveillance and clinical trials.

DNA, Bacterial, Haemophilus, Haemophilus Infections, High-Throughput Screening Assays, Humans, Molecular Diagnostic Techniques, Molecular Sequence Data, Polymerase Chain Reaction, Sensitivity and Specificity, Sequence Analysis, DNA, Transition Temperature
DNA, Bacterial, Haemophilus, Haemophilus Infections, High-Throughput Screening Assays, Humans, Molecular Diagnostic Techniques, Molecular Sequence Data, Polymerase Chain Reaction, Sensitivity and Specificity, Sequence Analysis, DNA, Transition Temperature
 
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