You are here: Home Published Research Th17 cells contribute to nontypeable Haemophilus influenzae-specific protective immunity induced by nasal vaccination with P6 outer membrane protein and α-galactosylceramide.

Kenji Noda, Satoru Kodama, Shingo Umemoto, Nozomi Nomi, Takashi Hirano, and Masashi Suzuki (2011)

Th17 cells contribute to nontypeable Haemophilus influenzae-specific protective immunity induced by nasal vaccination with P6 outer membrane protein and α-galactosylceramide.

Microbiology and immunology, 55(8):574–581.

Nasal vaccination is an effective therapeutic means of preventing upper respiratory infection. Recently, nasal vaccination with P6 outer membrane protein of nontypeable Haemophilus influenzae (NTHi) and alpha-galactosylceramide (α-GalCer) was reported to induce NTHi-specific protective immunity. The present study investigated the role of the Th17 cells induced by nasal vaccination. Mice were immunized with P6 and α-GalCer, and their P6-specific immune responses were examined. Cytokine-producing cells were analyzed by flow cytometry, and expression of cytokines in P6-specific CD4+ T cells was determined by reverse transcription-polymerase chain reaction. Bacterial challenges were performed with live NTHi. To examine the role of Th17 cells, bacterial clearance was also evaluated after interleukin (IL)-17 neutralization. P6-specific nasal wash immunoglobulin (Ig) A and serum IgG were increased after immunization with P6 and α-GalCer. Specific IgA-producing cells increased markedly in the nasal passages (NPs) of the immunized mice. In addition to P6-specific Th1 and Th2 cells, IL-17-producing Th17 cells were induced in the NPs and spleen. Bacterial clearance was enhanced by nasal vaccination. Interestingly, impaired NTHi clearance was shown after IL-17 neutralization. These findings suggest that nasal vaccination with P6 and α-GalCer is an effective regimen for the induction of NTHi-specific protective immunity in the upper respiratory tract. In addition to antigen-specific secretory-IgA, specific Th17 cells induced by nasal vaccination contribute to protection against NTHi.

Adjuvants, Immunologic, Administration, Intranasal, Animals, Antibodies, Bacterial, Bacterial Outer Membrane Proteins, Blood, CD4-Positive T-Lymphocytes, Cytokines, Disease Models, Animal, Flow Cytometry, Galactosylceramides, Haemophilus Infections, Haemophilus Vaccines, Haemophilus influenzae, Immunity, Immunoglobulin A, Immunoglobulin G, Mice, Mice, Inbred BALB C, Nasal Mucosa, Reverse Transcriptase Polymerase Chain Reaction, Rodent Diseases, Th17 Cells, Vaccination
Adjuvants, Immunologic, Administration, Intranasal, Animals, Antibodies, Bacterial, Bacterial Outer Membrane Proteins, Blood, CD4-Positive T-Lymphocytes, Cytokines, Disease Models, Animal, Flow Cytometry, Galactosylceramides, Haemophilus Infections, Haemophilus Vaccines, Haemophilus influenzae, Immunity, Immunoglobulin A, Immunoglobulin G, Mice, Mice, Inbred BALB C, Nasal Mucosa, Reverse Transcriptase Polymerase Chain Reaction, Rodent Diseases, Th17 Cells, Vaccination
 
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