You are here: Home Published Research Binding of human factor H to outer membrane protein P5 of non-typeable Haemophilus influenzae contributes to complement resistance.

Jeroen D Langereis, Marien I de Jonge, and Jeffrey N Weiser (2014)

Binding of human factor H to outer membrane protein P5 of non-typeable Haemophilus influenzae contributes to complement resistance.

Molecular microbiology, 94(1):89–106.

Non-typeable Haemophilus influenzae is an opportunistic pathogen of the human upper respiratory tract and is often found to cause inflammatory diseases that include sinusitis, otitis media and exacerbations of chronic obstructive pulmonary disease. To persist in the inflammatory milieu during infection, non-typeable H. influenzae must resist the antimicrobial activity of the human complement system. Here, we used Tn-seq to identify genes important for resistance to complement-mediated killing. This screen identified outer membrane protein P5 in evasion of the alternative pathway of complement activation. Outer membrane protein P5 was shown to bind human complement regulatory protein factor H directly, thereby, preventing complement factor C3 deposition on the surface of the bacterium. Furthermore, we show that amino acid variation within surface-exposed regions within outer membrane P5 affected the level of factor H binding between individual strains.

Bacterial Outer Membrane Proteins, Complement C3, Complement Factor H, Haemophilus Infections, Haemophilus influenzae, Host-Pathogen Interactions, Humans
Bacterial Outer Membrane Proteins, Complement C3, Complement Factor H, Haemophilus Infections, Haemophilus influenzae, Host-Pathogen Interactions, Humans
 
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