You are here: Home Published Research Resistance to complement-mediated killing and IgM binding to non-typeable Haemophilus influenzae is not altered when ascending from the nasopharynx to the middle ears in children with otitis media.

Jeroen D Langereis, Thijs MA van Dongen, Kim Stol, Roderick P Venekamp, Anne GM Schilder, and Peter WM Hermans (2013)

Resistance to complement-mediated killing and IgM binding to non-typeable Haemophilus influenzae is not altered when ascending from the nasopharynx to the middle ears in children with otitis media.

Medical microbiology and immunology, 202(6):407–415.

We have previously found that non-typeable Haemophilus influenzae (NTHi) collected from the middle ear of children with otitis media (OM) exhibit increased levels of complement resistance compared to NTHi collected from the nasopharynx. However, it is unknown whether bacteria develop complement resistance in the middle ear, or whether resistance is present when residing in the nasopharynx. The objective of this study was to investigate whether the levels of complement resistance of isolates collected from the middle ear were similar to those of isolates from the nasopharynx with an identical MLST type. We included 62 children with recurrent acute OM, chronic OM with effusion or acute tympanostomy tube otorrhea. NTHi was simultaneously isolated from the nasopharynx and middle ear fluid. MLST, resistance to complement-mediated killing, IgG binding, IgM binding and phosphorylcholine expression was determined. In 41 children, NTHi isolated from the middle ear and nasopharynx showed to have an identical MLST type. Isolates collected from the middle ear showed a highly similar level of complement resistance and IgM binding with isolates collected from the nasopharynx, whereas this was not the case for IgG binding and phosphorylcholine incorporation into lipooligosaccharide. Resistance to complement-mediated killing and IgM binding of NTHi isolates with an identical MLST type collected from the middle ear and nasopharynx of children with OM was highly similar.

Antibodies, Bacterial, Child, Child, Preschool, Cohort Studies, Complement System Proteins, DNA, Bacterial, Ear, Middle, Female, Haemophilus Infections, Haemophilus influenzae, Humans, Immunoglobulin G, Immunoglobulin M, Infant, Male, Microbial Viability, Multilocus Sequence Typing, Nasopharynx, Otitis Media, Phosphorylcholine
Antibodies, Bacterial, Child, Child, Preschool, Cohort Studies, Complement System Proteins, DNA, Bacterial, Ear, Middle, Female, Haemophilus Infections, Haemophilus influenzae, Humans, Immunoglobulin G, Immunoglobulin M, Infant, Male, Microbial Viability, Multilocus Sequence Typing, Nasopharynx, Otitis Media, Phosphorylcholine
 
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