You are here: Home Published Research Variation in expression of HMW1 and HMW2 adhesins in invasive nontypeable Haemophilus influenzae isolates.

Maria Giufrè, Alessandra Carattoli, Rita Cardines, Paola Mastrantonio, and Marina Cerquetti (2008)

Variation in expression of HMW1 and HMW2 adhesins in invasive nontypeable Haemophilus influenzae isolates.

BMC microbiology, 8:83.

Among surface antigens of nontypeable Haemophilus influenzae (NTHi), the HMW1 and HMW2 proteins are the major adhesins promoting colonization of the upper respiratory tract. Since they are potential vaccine candidates, knowledge concerning variation in HMW proteins expression among clinical isolates is of great interest. In this study, expression of hmw1A and hmw2A genes was evaluated by quantitative real-time reverse transcription-PCR in 3 NTHi invasive isolates (strains 56, 72, 91) and in the prototype strain 12. Number of 7-bp repeats within the hmwA promoters and presence of HMW proteins by Western blotting were also determined.

Adhesins, Bacterial, Gene Expression Regulation, Bacterial, Genetic Variation, Haemophilus influenzae, Promoter Regions, Genetic, RNA, Bacterial, Reverse Transcriptase Polymerase Chain Reaction, Tandem Repeat Sequences
Adhesins, Bacterial, Gene Expression Regulation, Bacterial, Genetic Variation, Haemophilus influenzae, Promoter Regions, Genetic, RNA, Bacterial, Reverse Transcriptase Polymerase Chain Reaction, Tandem Repeat Sequences
 
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