You are here: Home Published Research Epidemiology and evolution of antibiotic resistance of Haemophilus influenzae in children 5 years of age or less in France, 2001-2008: a retrospective database analysis.

H. Dabernat and C. Delmas (2012)

Epidemiology and evolution of antibiotic resistance of Haemophilus influenzae in children 5 years of age or less in France, 2001-2008: a retrospective database analysis.

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 31(10):2745–2753.

Trends in the evolution of antimicrobial resistance and mechanisms of resistance of Haemophilus influenzae to β-lactam antibiotics in France were assessed through a retrospective database review. The antimicrobial resistance of 2,206 H. influenzae strains from children aged ≤5 years was studied between 2001 and 2008. Strains were isolated from blood or cerebrospinal fluid (n = 170), bronchial secretions (n = 188), middle ear fluid, and nasopharynx or conjunctiva (n = 1,848). A proportion of 95.1 % (n = 2,097) were non-typeable H. influenzae (NTHi). β-lactamase production was identified in 27.5 % of NTHi isolates (all TEM-1), while β-lactamase-negative ampicillin resistance and β-lactamase-negative amoxicillin-clavulanate resistance among NTHi was 16.9 and 6.4 %, respectively. Over time, a statistically significant decrease in β-lactamase-producing strain prevalence (p < 0.0001) and a statistically significant increase in β-lactamase-negative ampicillin-resistant (BLNAR) strains (p < 0.0001) were observed in NTHi isolates from 2001 to 2008. The largest changes coincided with a campaign to reduce antibiotic use in France. An increasing diversity of amino acid substitution patterns was observed, with the emergence of group III/'III-like' patterns linked to high-level resistance. In France, amino acid substitution patterns are increasingly diverse, and strains with high-level antibiotic resistance are emerging. This study highlights the complexity of resistance dynamics within a given country. These results have implications on antibiotic guidelines and illustrate the importance of continued surveillance.

Amino Acid Substitution, Amoxicillin, Anti-Bacterial Agents, Bronchi, Child, Preschool, Conjunctiva, Databases, Factual, Drug Resistance, Multiple, Bacterial, Ear, Middle, Evolution, Molecular, France, Haemophilus Infections, Haemophilus influenzae, Humans, Infant, Microbial Sensitivity Tests, Nasopharynx, Population Surveillance, Prevalence, Retrospective Studies, beta-Lactamases, beta-Lactams
Amino Acid Substitution, Amoxicillin, Anti-Bacterial Agents, Bronchi, Child, Preschool, Conjunctiva, Databases, Factual, Drug Resistance, Multiple, Bacterial, Ear, Middle, Evolution, Molecular, France, Haemophilus Infections, Haemophilus influenzae, Humans, Infant, Microbial Sensitivity Tests, Nasopharynx, Population Surveillance, Prevalence, Retrospective Studies, beta-Lactamases, beta-Lactams
 
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