You are here: Home Published Research Identification of Haemophilus influenzae and Haemophilus haemolyticus by matrix-assisted laser desorption ionization-time of flight mass spectrometry.

J. P Bruin, M. Kostrzewa, A. van der Ende, P. Badoux, R. Jansen, S. A Boers, and B. MW Diederen (2014)

Identification of Haemophilus influenzae and Haemophilus haemolyticus by matrix-assisted laser desorption ionization-time of flight mass spectrometry.

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 33(2):279–284.

Generally accepted laboratory methods that have been used for decades do not reliably distinguish between H. influenzae and H. haemolyticus isolates. H. haemolyticus strains are often incorrectly identified as nontypeable Haemophilus influenzae (NTHi). To distinguish H. influenzae from H. haemolyticus we have created a new database on the matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) bio-typer 2 and compared the results with routine determination of Haemophilus (growth requirement for X and V factor), and multilocus sequence typing (MLST). In total we have tested 277 isolates, 244 H. influenzae and 33 H. haemolyticus. Using MLST as the gold standard, the agreement of MALDI-TOF MS was 99.6 %. MALDI-TOF MS allows reliable and rapid discrimination between H. influenzae and H. haemolyticus.

Bacteriological Techniques, Haemophilus, Haemophilus Infections, Humans, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Bacteriological Techniques, Haemophilus, Haemophilus Infections, Humans, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
 
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